7,350 research outputs found

    Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe

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    Background DNA replication initiates on defined genome sites, termed origins. Origin usage appears to follow common rules in the eukaryotic organisms examined to date: all chromosomes are replicated from multiple origins, which display variations in firing efficiency and are selected from a larger pool of potential origins. To ask if these features of DNA replication are true of all eukaryotes, we describe genome-wide origin mapping in the parasite Leishmania. Results Origin mapping in Leishmania suggests a striking divergence in origin usage relative to characterized eukaryotes, since each chromosome appears to be replicated from a single origin. By comparing two species of Leishmania, we find evidence that such origin singularity is maintained in the face of chromosome fusion or fission events during evolution. Mapping Leishmania origins suggests that all origins fire with equal efficiency, and that the genomic sites occupied by origins differ from related non-origins sites. Finally, we provide evidence that origin location in Leishmania displays striking conservation with Trypanosoma brucei, despite the latter parasite replicating its chromosomes from multiple, variable strength origins. Conclusions The demonstration of chromosome replication for a single origin in Leishmania, a microbial eukaryote, has implications for the evolution of origin multiplicity and associated controls, and may explain the pervasive aneuploidy that characterizes Leishmania chromosome architecture

    Low Reynolds Number Aerodynamic Characteristics of Several Airplane Configurations Designed to Fly in the Mars Atmosphere at Subsonic Speeds

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    A 1/4-scale wind tunnel model of an airplane configuration developed for short duration flight at subsonic speeds in the Martian atmosphere has been tested in the Langley Research Center Transonic Dynamics Tunnel. The tunnel was pumped down to extremely low pressures to represent Martian Mach/Reynolds number conditions. Aerodynamic data were obtained and upper and lower surface wind pressures were measured at one spanwise station on some configurations. Three unswept wings of the same planform but different airfoil sections were tested. Horizontal tail incidence was varied as was the deflection of plain and split trailing-edge flaps. One unswept wing configuration was tested with the lower part of the fuselage removed and the vertical/horizontal tail assembly inverted and mounted from beneath the fuselage. A sweptback wing was also tested. Tests were conducted at Mach numbers from 0.50 to 0.90. Wing chord Reynolds number was varied from 40,000 to 100,000 and angles of attack and sideslip were varied from -10deg to 20deg and -10deg to 10deg, respectively

    DNA Fingerprinting

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    The purpose of this IQP was to trace the development and progress of DNA fingerprinting technology as it continues to be a integral part of judicial systems and societies world-wide. Much of the initial DNA fingerprinting methods and techniques have undergone substantial improvements to satiate the bombardment of skeptics, giving the technology more credibility in courts. However, there is still the unresolved issue of privacy laws that continue to complicate the issue of who should submit samples to DNA databases

    The He abundance in the metal-deficient blue compact dwarf galaxies Tol 1214-277 and Tol 65

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    We present high-quality Keck telescope spectroscopic observations of the two metal-deficient blue compact dwarf (BCD) galaxies Tol 1214-277 and Tol 65. These data are used to derive the heavy-element and helium abundances. We find that the oxygen abundances in Tol 1214-277 and Tol 65 are the same, 12+logO/H=7.54+/-0.01, or Zsun/24, despite the different ionization conditions in these galaxies. The nitrogen-to-oxygen abundance ratio in both galaxies is logN/O=-1.64+/-0.02 and lies in the narrow range found for the other most metal-deficient BCDs. We use the five strongest HeI emission lines 3889, 4471, 5876, 6678 and 7065, to correct self-consistently their intensities for collisional and fluorescent enhancement mechanisms and to derive the He abundance. Underlying stellar absorption is found to be important for the HeI 4471 emission line in both galaxies, being larger in Tol 65. The weighted He mass fractions in Tol 1214-277 and Tol 65 are respectively Y=0.2458+/-0.0039 and 0.2410+/-0.0050 when the three HeI emission lines, 4471, 5876 and 6678, are used, and are, respectively, 0.2466+/-0.0043 and 0.2463+/-0.0057 when the HeI 4471 emission line is excluded. These values are in very good agreement with recent measurements of the He mass fraction in others of the most metal-deficient BCDs by Izotov and coworkers. We find that the combined effect of the systematic uncertainties due to the underlying HeI stellar absorption lines, ionization and temperature structure of the HII region and collisional excitation of the hydrogen emission lines is likely small, not exceeding ~2% (the error is 2sigma). Our results support the validity of the standard big bang model of nucleosynthesis.Comment: 22 pages, 3 Postscript figures, accepted for publication in the Astrophysical Journa

    Mobile satellite propagation measurements and modeling: A review of results for systems engineers

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    An overview of Mobile Satellite Service (MSS) propagation measurements and modeling is intended as a summary of current results. While such research is on-going, the simple models presented here should be useful to systems engineers. A complete summary of propagation experiments with literature references is also included
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